adiponectin levels  (R&D Systems)

Journal: BioMed Research International

Article Title: Galectin‐1, Adiponectin, Leptin, and the Adiponectin to Leptin Ratio as Predictors of Metabolic Syndrome: Influence of Age and Body Mass Index

doi: 10.1155/bmri/1789457

Figure Lengend Snippet: Comparison of galectin‐1, leptin, adiponectin, and adiponectin/leptin ratio between metabolic syndrome and control obese participants.

Article Snippet: Galectin‐1 and adiponectin levels were significantly lower in the MetS group, whereas leptin levels were not significantly higher.

Techniques: Comparison, Control

Journal: Journal of Animal Science and Biotechnology

Article Title: Hesperidin alleviates systemic inflammation and oxidative stress by remodeling adipose tissue lipid metabolism in periparturient dairy cows

doi: 10.1186/s40104-026-01372-4

Figure Lengend Snippet: Changes on serum glucose and lipid metabolism indices in periparturient cows fed with hesperidin. A NEFA; B BHB; C glucose; D insulin; E TG; F TC; G adiponectin; H RQUCKI. A total of 32 cows were assigned to 1 of 2 dietary treatments (TMR with or without HES) in a randomized complete block design. Serum indices were measured on d −21, −14, −7, −1, 3, 10, 20, and 30 relative to calving. CON, no supplemental hesperidin; HES, 30 g/d hesperidin; Trt, treatment; NEFA, non-esterified fatty acid; BHB, β-hydroxybutyrate; TG, triglyceride; TC, total cholesterol; RQUICKI, revised quantitative insulin sensitivity check index. Error bars represent SE of the LSM

Article Snippet: Levels of insulin (INS; #H203-1-1), adiponectin (#H179-1–2), IL-1β (#H002-1–2), IL-2 (#H003-1-1), IL-6 (#H007-1-2), IL-18 (#H015-1-2), TNF-α (#H052-1-2), serum amyloid A (SAA; #H134), lipopolysaccharide-binding protein (LBP; #H253-1-2), caspase-1 (Cas-1; #H074-1-2), and apoptosis-associated speck-like protein containing a CARD (ASC; #H597) were quantified using bovine-specific enzyme-linked immunosorbent assay (ELISA) kits (Nanjing Jiancheng Bioengineering Institute, Nanjing, China), based on antigen-antibody interactions and subsequent colorimetric detection.

Techniques: Blocking Assay

Journal: Journal of Animal Science and Biotechnology

Article Title: Hesperidin alleviates systemic inflammation and oxidative stress by remodeling adipose tissue lipid metabolism in periparturient dairy cows

doi: 10.1186/s40104-026-01372-4

Figure Lengend Snippet: Changes on adipose tissue antioxidant status and adiponectin in periparturient cows fed with hesperidin. A T-AOC; B SOD; C adiponectin. A total of 32 cows were assigned to 1 of 2 dietary treatments (TMR with or without HES) in a randomized complete block design. Adipose tissue was biopsied on d 5, 10, and 25 relative to calving. CON, no supplemental hesperidin; HES, 30 g/d hesperidin; Trt, treatment; T-AOC, total antioxidant capacity; SOD, superoxide dismutase. Error bars represent SE of the LSM. If interactions between treatment and time were significant ( P < 0.05), data were analyzed individually for each time point using t -test ( * P < 0.05; * * P < 0.01; *** P < 0.001)

Article Snippet: Levels of insulin (INS; #H203-1-1), adiponectin (#H179-1–2), IL-1β (#H002-1–2), IL-2 (#H003-1-1), IL-6 (#H007-1-2), IL-18 (#H015-1-2), TNF-α (#H052-1-2), serum amyloid A (SAA; #H134), lipopolysaccharide-binding protein (LBP; #H253-1-2), caspase-1 (Cas-1; #H074-1-2), and apoptosis-associated speck-like protein containing a CARD (ASC; #H597) were quantified using bovine-specific enzyme-linked immunosorbent assay (ELISA) kits (Nanjing Jiancheng Bioengineering Institute, Nanjing, China), based on antigen-antibody interactions and subsequent colorimetric detection.

Techniques: Blocking Assay

Journal: Journal of Animal Science and Biotechnology

Article Title: Hesperidin alleviates systemic inflammation and oxidative stress by remodeling adipose tissue lipid metabolism in periparturient dairy cows

doi: 10.1186/s40104-026-01372-4

Figure Lengend Snippet: Proposed mechanisms by which hesperidin improves sphingolipid metabolism and metabolic health in adipose tissue of periparturient cows. A Integrated lipidomic and proteomic analysis of adipose tissue sphingolipid metabolism. Differential metabolites and proteins are mapped to the de novo biosynthesis, salvage, and hydrolysis pathways. Hesperidin supplementation led to the downregulation of key ceramide synthases (CERS2, CERS5, CERS6) and ceramide accumulation, while promoting sphingomyelin synthesis (SGMS1/2) and hydrolysis (SMPD1/3). These coordinated changes indicate reduced ceramide burden and enhanced sphingolipid remodeling. B Proposed working model of hesperidin action in adipose tissue and systemic metabolism. Hesperidin-derived metabolites accumulate in adipose tissue, where they promote PPARγ activation and adiponectin secretion, while attenuating ceramide-driven ER stress, inflammasome activation (NLRP3–caspase-1), oxidative stress, and lipolysis. These changes improve insulin signaling and glucose uptake, lower circulating NEFA and BHBA, and ultimately alleviate systemic oxidative stress and inflammation.

Article Snippet: Levels of insulin (INS; #H203-1-1), adiponectin (#H179-1–2), IL-1β (#H002-1–2), IL-2 (#H003-1-1), IL-6 (#H007-1-2), IL-18 (#H015-1-2), TNF-α (#H052-1-2), serum amyloid A (SAA; #H134), lipopolysaccharide-binding protein (LBP; #H253-1-2), caspase-1 (Cas-1; #H074-1-2), and apoptosis-associated speck-like protein containing a CARD (ASC; #H597) were quantified using bovine-specific enzyme-linked immunosorbent assay (ELISA) kits (Nanjing Jiancheng Bioengineering Institute, Nanjing, China), based on antigen-antibody interactions and subsequent colorimetric detection.

Techniques: Derivative Assay, Activation Assay